MS Report from Instrument: HY-LCMS-02 File D:\\CHEM32\\1\\DATA\\BIZ\\BIZ2017-217-WJ8-2.D Tgt Mass (EZX): 616.40 Injection Date : 17 Feb 17 1:52 pm +0800 Seq. Line : 0 Sample Name : BIZ2017-217-WJ8-2 Location : P1-B-03 Acq. Operator : GZZ_718 Inj : 1 Spec. Reported : MS Integration Inj Volume : 20 ul Acq. Method : D:\\Chem32\\1\\METHODS\\1-POS-3MIN.M Analysis Method : D:\\CHEM32\\1\\METHODS\\1-POS-3MIN-PROSRSS.M Catalog No : HY-18739 Batch#23560 Easy-Access Method: '1-POS-3MIN' 616.40 Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min Column :SunFire C18, 4.6*50mm,3.5um A-RP-186 Oven Temperature : 45C*DAD1 B, Sig=214,4 Ref=off mAU4001.3721.5091.7151.7992.2732.3251.9392.4342.56120000.5*DAD1 C, Sig=254,4 Ref=off mAU3002002.3502.4571.5091.6631.69210000.51 MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 7012500010000075000500002500001.9622.0122.1322.55812.8721.522.52.8731.522.5min*1.3731.5452.89522.5min1.522.5min0.511.5 Ion 617.4, MSD1 617.4,Target Mass 616.4 +H Positive, EIC=617.1:618.1 2001501005000.5 Integration Results for DAD1 B, Sig=214,4 Ref=off1min* RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.37 0.04 12.73 5.95 0.59 340 1.51 0.02 6.86 5.09 0.32 274 1.72 0.03 3.92 2.41 0.18 212 1.80 0.03 2.50 1.57 0.11 279 1.94 0.04 3.90 0.03 0.18 233 2.27 0.02 2.18 1.36 0.10 233 2.32 0.03 2.85 0.32 0.13 141 2.43 0.03 2.33 -0.13 0.11 141 2.56 0.04 5.39 2.50 0.25 141 2.87 0.05 2130.07 655.38 98.04 311 Integration Results for DAD1 C, Sig=254,4 Ref=off RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 1.51 0.02 4.42 3.31 0.36 274 1.66 0.02 0.49 0.36 0.04 302 1.69 0.02 1.04 -0.02 0.08 212 1.96 0.02 1.31 0.05 0.11 233HY-LCMS-02 2/17/2017 2:20:29 PM GZZ_718Page 1 of 3
MS Report from Instrument: HY-LCMS-02
2.01 0.03 0.98 0.64 0.08 233 2.13 0.03 3.54 2.09 0.29 233 2.35 0.03 9.53 4.82 0.77 141 2.46 0.03 8.96 4.30 0.73 141 2.56 0.04 8.48 3.52 0.69 141 2.87 0.05 1196.11 370.57 96.86 311HY-LCMS-02 2/17/2017 2:20:29 PM GZZ_718Page 2 of 3
MS Report from Instrument: HY-LCMS-02
Ret. Time: 1.37 <<<< POSITIVE SPECTRA >>>> ES-API Positive 396.9140012001000 701.51600 340.3800600 680.3 703.4 702.5 792.4 815.5 814.5 218.3 397.3 453.3 141.0 262.1 460.4 509.3 566.3 116.2 302.5 333.1 227.4 372.24002000 421.1 592.5 927.5900m/z900m/z900m/z200300400500600700800Ret. Time: 1.54 ES-API Positive500040003000 141.2200010000 233.1 274.4 276.2200300 318.2400 455.1500600700800Ret. Time: 2.90 ES-API Positive 311.3200001750015000125007500500025000 141.3 233.2 312.3 353.2 390.110000 389.3200300400500600 640.3700800HY-LCMS-02 2/17/2017 2:20:29 PM GZZ_718Page 3 of 3