pGEX-6P-1大肠杆菌表达载体说明

编号\r  

北京华越洋生物VECT4980\r  

载体名称\r  

pGEX-­‐6P-­‐1\r  

\r  

pGEX6P1载体基本信息\r  别名:\r  pGEX-­‐6P-­‐1,\r  pGEX6P1,\r  pGEX\r  6P\r  1\r  质粒类型:\r  大肠杆菌蛋白表达载体\r  表达水平:\r  高拷贝\r  启动子:\r  Tac\r  克隆方法:\r  多克隆位点，限制性内切酶\r  载体大小:\r  4984\r  bp\r  5'\r  测序引物及序列:\r  pGEX5':\r  \r  GGGCTGGCAAGCCACGTTTGGTG\r  3'\r  测序引物及序列:\r  pGEX3':\r  CCGGGAGCTGCATGTGTCAGAGG\r  载体标签:\r  N-­‐GST\r  载体抗性:\r  Ampicillin\r  氨苄\r  备注:\r  复制子是pMB1\r  稳定性:\r  瞬时表达\r  Transient\r  组成型:\r  诱导表达\r  病毒/非病毒:\r  非病毒\r  \r  

pGEX6P1载体质粒图谱和多克隆位点信息\r  

、\r  

\r  

pGEX6P1载体序列\r  

ORIGIN

1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG 61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT 121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC 181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA 241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC 301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC 361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC 421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA 481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC 541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT 601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG 661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT 721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA 781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT 841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC 901 ATCCTCCAAA ATCGGATCTG GAAGTTCTGT TCCAGGGGCC CCTGGGATCC CCGGAATTCC 961 CGGGTCGACT CGAGCGGCCG CATCGTGACT GACTGACGAT CTGCCTCGCG CGTTTCGGTG 1021 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 1081 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 1141 GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TAATTCTTGA AGACGAAAGG 1201 GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT 1261 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 1321 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA 1381 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT 1441 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC 1501 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 1561 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 1621 CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC 1681 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG 1741 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC 1801 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 1861 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 1921 ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC 1981 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC 2041 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 2101 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG 2161 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 2221 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC 2281 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 2341 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 2401 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 2461 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 2521 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT

2581 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 2641 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 2701 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 2761 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 2821 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 2881 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 2941 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 3001 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 3061 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 3121 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG 3181 AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC 3241 ACCGCATAAA TTCCGACACC ATCGAATGGT GCAAAACCTT TCGCGGTATG GCATGATAGC 3301 GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG 3361 CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG 3421 TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA 3481 ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA 3541 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC 3601 TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG 3661 CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG 3721 ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG 3781 TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG 3841 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA 3901 GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT CGCAATCAAA 3961 TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT CAACAAACCA 4021 TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG 4081 CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GATATCTCGG 4141 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTCA ACCACCATCA 4201 AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG 4261 GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA AAAACCACCC 4321 TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 4381 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG 4441 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA 4501 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGGATTCACT 4561 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT 4621 TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC 4681 TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA 4741 AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC 4801 CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTAA CCTATCCCAT 4861 TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT 4921 TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTTGG 4981 AATT

//\r  \r  

其他大肠杆菌表达载体：

pBV221

pDsRed-Express2 pCold TF pCold I

pBAD202/D-TOPO pBad/Myc-His B ptdTomato pBV220 pCold IV pE-SUMO pACYC184 pET-52b(+) pCold-GST pCold III pCold-ProS2 pBAD/Thio-TOPO pAmCyan pColdS-SUMO pCold II pBAD102/D-TOPO pBad/Myc-His C pBad/His B pBad/Myc-His A pBad/His C

pBad/His A pET-23c(+) pET-12a(+) pBad24 pQE-32 pQE-60 pET-50b(+) pET-26b(+) pET-14b pET-20b(+) pET-21a(+) pET-27b(+) pET-43.1c(+) pET-44c(+) pTrcHis2 B pRSET-CFP pET300/NT-DEST pGEX-4T-3 pG-KJE8 pET-17b pTf16 pSUMO pKD3 pTYB2 pMAL-p5e PinPoint Xa-3 pSP64 pET-5b(+) pET-5a(+) pkk232-8 pMAL-c5x

pET-43.1 EK/LIC pET-31b(+) pGEX-4T-2 pET-28b(+) pBR322

pEcoli-6xHN-GFPuv pBAD-TOPO pET-23(+) pET-11b(+) pQE-82L pQE-9 pQE-70 pET-49b(+) pET-32a(+) pET-16b pET-21d(+) pET-24a(+) pET-28a(+) pET-43.1b(+) pET-46 EK/LIC pTrcHis2 A pRSET-EmGFP pET301/CT-DEST pGEX-5X-2 pGro7

pET102/D-TOPO pET-28c(+) pProEX HTc pKD13 pTWIN2 pACYCDuet-1 PinPoint Xa-2 pTWIN1 pBad/gIII C pMal-p4X pCYB1 pMal-p2E pET-41 EK/LIC pET-3b(+) pETDuet-1 pET-His pKD4

pKJE7

pET-23b(+) pET-12b(+) pET-11a(+) pQE-81L pQE-16 pQE-40 pET-48b(+) pET-21b(+) pET-15b pET-21c(+) pET-24d(+) pET-30a(+) pET-43.1a(+) pET-37b(+) pET303/CT-His pRSET-BFP

pGEM-T

pBlueScript SK(+) pET-SUMO pCDFDuet-1 pBluescript II SK(+) pProEX HTb pKD46

pBluescript II KS(-) pEGM-11ZF(+) PinPoint Xa-1 pTYB11 pBad/gIII B pMal-p2G pEZZ18 pMal-p2X pMal-c4X pET-41a(+) pGEX-4T-1 pALEX a,b,c pKD20 pRSET B

pET-23a(+) pET-12c(+) pET-11c(+) pQE-80L pQE-31 pET-51b(+) pET-47b(+) pET-22b(+) pET-19b pET-21b(+) pET-25b(+) pET-42a(+) pET-44a(+) pTrcHis2 C pET302/NT-His pGFPuv pBad43 pG-Tf2 pSE380 pMAL-p5x pET-30b(+)

pProEX HTa pTYB1 pTYB12

pEGM-7ZF(+) pSP73 pTXB1 pBad/gIII A pkk223-3 pBAD18

pET-44 EK/LIC pTrcHis B pGEX-3X pTrc99a pACYC177 pMXB10 pGEX-KG

pGEX-2T pTrcHis A pGEX-6P-3 pGEX-5X-1 pMal-c2E pET-45b(+) pET-40b(+) pET-32 Xa/LIC pET-30 EK/LIC pET-29a(+) pET-23d(+)

pRSFDuet-1 pET-41b(+) pGEX-6P-2 pGEX-2TK pMal-c2X pET-44b(+) pET-33b(+) pET-32c(+) pET-30c(+) pET-24c(+) pET-11d(+)

pCOLADuet-1 pET-42b(+) pGEX-6P-1 pRSET A pRSET C pET-42c(+) pET-39b(+) pET-32b(+) pET-29c(+) pET-24b(+) pBad33

pTrcHis C pET-3a(+) pGEX-5X-3 pMal-c2G pQE-30 pET-41c(+) pET-32 EK/LIC pET-30 Xa/LIC pET-29b(+) pET-24(+)

\r