pGL3-Enhancer Vector
The pGL3-Enhancer Vector contains an SV40 enhancer located downstream of
luc+ and the poly(A) signal. This aids in the verification of functional promoter
elements because the presence of an enhancer will often result in transcription
of luc+ at higher levels.
pGL3-Enhancer Vector Sequence Reference Points:
Promoter (none)
Multiple cloning region 1–58
Luciferase gene (luc+) 88–1740
GLprimer2 binding site 89–111
SV40 late poly(A) signal 1772–1993
Enhancer 2013–2249
RVprimer4 binding site 2307–2326
ColE1-derived plasmid replication origin 2564
β-lactamase gene (Ampr) 3329–4186
f1 origin 4318–4773
upstream poly(A) signal 4904–5057
RVprimer3 binding site 5006–5025
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